副教授

张文

张文(1971~),副研究员,研究生导师


个人简介:

       华中科技大学研究生,中科院生化所和伯克利大学学习工作。主要研究领域:通过结构生物学和生物信息学,研究核糖体的结构功能和抗生素作用机制。代表性成果:参与第一个核糖体70S的高精度结构模建与解析;解析第一个高精度的翻译延长中的重要动态中间态结构,解释了翻译延长过程;研究了十多个抗生素复合物结构,参与几个新型抗生素研发;运用计算进行结构模型构建、生物信息研究,并开发一个晶体数据辅助处理的程序包。近年发表研究论文近十篇,其中第一作者在《Science》、《Nature Struct Mol Biol》等发表;另参与《Science》、《PNAS》、《J Clin Invest》等论文,总索引数超过1200。目前有2个重要的研究成果正在整理最后数据,有待近期送审。获973、国家自然科学基金项目2项,经费100余万元,参与申请并获得国际专利一项。


研究方向:
1. Ribosome structure and dynamics during translation elongation 
2. Ribosome-antibiotics complex and drug resistance mechanism


代表性论文:
1. Y Ruan, L Sun, Y Hao, L Wang, J Xu, W Zhang,et al. Ribosomal RACK1 promotes chemoresistance and growth in human hepatocellular carcinoma. J Clin Invest. 2012; 122:2554–2566 (initial data for crystallography. )
2. Beatriz LS, Dunkle J, Dorota L, Zhang W, Fernandes P, Cate J, Alexander M. Binding and action of CEM-101, a new fluor oketolide antibiotic that inhibits protein synthesis. Antimicrobial Agents and Chemotherapy. 2010, Vol. 54: 4961-4970 
3. Zhang W*, Dunkle J, Cate J. Structures of the ribosome in intermediate states of ratcheting. Science 325, 1014 (2009). True milestone work comments by Liljas Anders. Leaps in Translational Elongation. Science 326, 677-678 
4. Pai RD*, Zhang W*, Schuwirth BS, Hirokawa G, Kaji H, Kaji A, Cate JHD. Structural Insights into Ribosome Recycling Factor Interactions with the 70S Ribosome. J Mol Biol. 2008 7; 376(5):1334-47. *Equal contribution  
5. Borovinskaya MA*, Pai RD*, Zhang W*, Schuwirth BS, Holton JM, Hirokawa G, Kaji H, Kaji A, Cate JH. Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Nature Struct Mol Biol. 2007 Aug; 14(8):727-32.  (Important Progress cited by APS 2007 annual report ) *Equal contribution 
6. Berk V, Zhang W, Pai RD, Cate JH. Structural basis for mRNA and tRNA positioning on the ribosome. Proc Natl Acad Sci USA. 2006 Oct 24; 103(43): 15830-4 
7. Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Sanjurjo AV, Holton JM , Cate JHD, Structures of the bacterial ribosome at 3.5 Å resolution. Science 2005 4; 310: 827-34    
8. Anton VS, Ridgeway W, Seymaner V, Zhang W, Santoso S, Yu K,Cate JHD. X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli. Proc Natl Acad Sci USA 100(15) P8682 

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